Hormonal Testing: Hormonal Analysis in Moscow

Most chronic complaints without an obvious cause are caused by a hormonal imbalance—and it can be corrected if detected early. K+31 endocrinologists will help you determine which tests are necessary for your situation and explain the results during your appointment.

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Why get tested for hormones?

Hormones are biologically active substances that regulate virtually all processes in the body: metabolism, reproductive function, growth, weight, mood, sleep, and immune response. Hormonal imbalances often develop gradually and go unnoticed for a long time, disguised as chronic fatigue, weight gain, menstrual irregularities, or decreased libido.

A hormonal test is a blood test that measures the levels of specific hormones or a panel of hormones. This is not the same as a biochemical blood test (ALT, AST, cholesterol): biochemical blood tests reflect organ function, while hormonal panels reflect the function of the endocrine glands.

Why get tested for hormones?

Basic Hormonal Panels: What, Why, and When

Thyroid gland

The most common reason for referral to an endocrinologist.

Indicator What it reflects Indications
TSH (thyroid-stimulating hormone) A regulatory signal from the pituitary gland to the thyroid gland; Main screening marker Screening, fatigue, weight change, menstrual irregularities
Free T4 (fT4) Active form of thyroxine Clarification of diagnosis with changes in TSH
Free T3 (fT3) The most active thyroid hormone Therapy monitoring, suspected T3 thyrotoxicosis
Anti-TPO Anti-thyroid peroxidase antibodies Suspected autoimmune Hashimoto's thyroiditis
Anti-TG Anti-thyroglobulin antibodies Additional diagnostics of autoimmune thyroiditis, monitoring after thyroid surgery
TG (thyroglobulin) Thyroid tissue marker Treatment monitoring for differentiated thyroid cancer
Calcitonin Thyroid C-cell marker Screening for medullary thyroid cancer

Pituitary

The main "conductor" gland, regulating the thyroid, adrenal, and gonads.

Indicator What it reflects Indications
Prolactin Lactotropic hormone Irregularities in menstrual cycles, galactorrhea, decreased libido, infertility, suspected prolactinoma
LH (luteinizing hormone) Stimulates Ovulation in women, testosterone synthesis in men Menstrual cycle disorders, fertility assessment
FSH (follicle-stimulating hormone) Regulates follicle maturation and spermatogenesis Menstrual cycle disorders, male infertility, ovarian reserve assessment
GH (somatotropic hormone) Growth hormone Diagnosis of acromegaly or GH deficiency
ACTH Stimulates the adrenal glands Diagnosis of Cushing's syndrome or adrenal insufficiency insufficiency

Adrenal glands

Indicator What it reflects Indications
Cortisol (morning, 24-hour urine or saliva) Stress and metabolic hormone Suspected Cushing's syndrome or adrenal insufficiency
DHEA-S Androgenic function of the adrenal cortex Signs of hyperandrogenism, menstrual irregularities, acne
Aldosterone + renin Regulation of water and salt balance Arterial hypertension, hypokalemia
Metanephrines (plasma or urine) Pheochromocytoma markers Paroxysmal hypertension, adrenal incidentaloma on CT scan

Sex Hormones

In women:

Indicator What it reflects Indications
Estradiol (E2) The main estrogen of reproductive age Cycle irregularities, menopausal symptoms, HRT assessment
Progesterone Luteal Phase Assessment Cycle Irregularities, Habitual Miscarriage, Anovulation
Total and Free Testosterone Androgen Status Acne, Hirsutism, Decreased Libido, PCOS
AMH (Anti-Müllerian Hormone) Ovarian Reserve Fertility Assessment, IVF Planning
HCG Pregnancy Hormone Pregnancy Confirmation, Ectopic Diagnosis, Monitoring

For men:

Indicator What it reflects Indications
Testosterone (total, free, bioavailable) Androgen status Decreased libido, erectile dysfunction, depression, osteoporosis in men
SHBG Steroid transfer protein Interpretation Testosterone
LH, FSH Pituitary regulation Male infertility, hypogonadism

General information

Parathyroid Hormones and Calcium Metabolism

Indicator What it Reflects Indications
Parathyroid Hormone (PTH) Regulation of Calcium and Phosphorus Hypercalcemia, Osteoporosis, Nephrolithiasis
Ionized Calcium Physiologically Active Fraction Hypercalcemia Clarification
Vitamin D (25-OH) Vitamin D Status Osteoporosis, fatigue, immune disorders, screening in risk groups
Parathyroid hormones and calcium metabolism

Insulin and the Regulation of Carbohydrate Metabolism

Indicator What it Reflects Indications
Fasting Insulin Basal Secretion Insulin Resistance, Hypoglycemia
C-Peptide Pancreatic β-Cell Secretory Function (Endogenous) Differential Diagnosis of Diabetes Types, Assessment of Reserve Function
HOMA-IR Insulin Resistance Index (calculated) Metabolic Syndrome Screening
Insulin and regulation of carbohydrate metabolism

Preparation Guidelines for Hormonal Testing

Hormonal levels depend on the time of day, phase of the menstrual cycle, diet, stress, and physical activity. Failure to follow the preparation rules will result in unreliable results.

General rules:

  • Blood is collected on an empty stomach (8-12 hours of fasting), only water
  • Avoid intense physical activity and stress the day before
  • Do not drink alcohol for 24 hours
  • Sex hormones are collected strictly on specific days of the cycle (see below)
  • If you are taking hormonal medications, check with your endocrinologist to see if you need to stop them

Days of the cycle for tests in women:

  • FSH, LH, estradiol, AMH, inhibin B: days 2-4 of the cycle
  • Progesterone: days 21-23 of the cycle (mid-cycle) Luteal phase)
  • Testosterone, DHEA-S, cortisol, TSH: any day, preferably morning

Special conditions:

  • Prolactin: avoid stress and physical activity before testing; for repeat testing, the same laboratory
  • Cortisol: strictly in the morning (8:00–9:00), at rest
  • Testosterone in men: in the morning, from 7:00 to 10:00
Rules for preparing for hormone testing

How does an endocrinologist consultation proceed based on results?

It's important to understand that hormonal tests are always interpreted in the context of symptoms, other indicators, and ultrasound and CT data. Independently interpreting "normal" and "abnormal" values ​​based on laboratory reference values ​​without taking clinical data into account often leads to erroneous conclusions.

During the appointment, endocrinologist K+31:

  1. Analyzes the results in the context of the patient's complaints and medical history.
  2. Determines the need for additional testing (ultrasound, CT, MRI).
  3. Prescribes treatment or observational therapy.
  4. Explains the meaning of each indicator.
How does an endocrinologist consultation proceed based on the results?

Frequently Asked Questions (FAQ)

What does "normal" mean in a hormone test?

Laboratory reference values ​​represent the range of values ​​found in 95% of generally healthy individuals. However, "normal" varies from person to person: borderline values ​​ in combination with symptoms may require treatment, while minor deviations without symptoms do not.

How different are the results of tests for the same hormone in different laboratories?

Different laboratories use different methods and reagents, so absolute values ​​may vary significantly. Dynamic monitoring should always be performed in a single laboratory.

Should I stop taking thyroxine (levothyroxine) before testing my TSH?

No. TSH reflects the pituitary gland's regulatory response, which changes slowly (over days to weeks). Missing a single pill or taking one in the morning before a TSH test has virtually no effect on the results.

Can stress and fatigue change hormonal levels?

Yes, especially cortisol (increases), prolactin (increases), sex hormones (decreases with chronic stress). Therefore, an acute illness or stress the day before is a reason to reschedule scheduled tests.

How often should I check my thyroid hormones?

For healthy individuals, TSH testing should be performed once every 3-5 years (or when symptoms appear). If a thyroid disorder has been diagnosed or if hormones are being taken, it should be performed according to the endocrinologist's recommendations (usually 1-2 times a year).

Can I drink water before taking tests?

Yes, you can drink plain still water. Coffee, tea, and juices—no.

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